猪“瘦肉精”检测方法的比较和应用/Contrast and Application of Detecting Clenbuterol-HCl Methods
Along with the development of society economy and the people’s living level, the consumption and yield of animal origin foodstuffs, such as meat, egg and milk, was stepping up rapidly. In latest years, the food poisoning events happened occasionally because the pesticide and veterinary drug were slathered in animal production. Thereinto the Clenbuterol-HCl residues had become the most serious in pig production. It had brought the worldwide attention.
Clenbuterol-HCl was a synthetic drug, which was used to treated asthma or beast bronchus as bronchia expending drug in human clinic medicine firstly. But later, it was widely used in animal feed as additives because it can improve the rate of lean meat in animal production. But it had result in a series of poisoning symptom when people eat the meat included the Clenbuterol-HCl. So China and other country had forbidden the Clenbuterol-HCl to feed animal as additive.
At present, our country had draw the correlative law and criterion for detecting Clenbuterol-HCl, but in fact, the events of masculine testing Clenbuterol-HCl in pig or meat and feed, and food poisoning had happened occasionally. Therefore, detection and restrict using the Clenbuterol-HCl in headstream become more important.
Now the main method to detect Clenbuterol-HCl are high performance liquid chromatography (HPLC), GC-MS, ELISA and rapid test paper. The former three methods are not unfit for the field and rapid test. The later method developed very fast in latest few years, but the quality of the rapid test paper produced by several factory was uneven. So the detecting effect was affected a certain extent.
The aim to find a rapid and accuracy method of detecting Clenbuterol-HCl, this study contrast several methods. The results showed that:
(1) HPLC, GC-MS and ELISA are reproducible and sensitive to Clenbuterol-HCl, the rapid test paper produced by DaLian Puruikang company among three is the most correct and exact method.
(2) The former three methods need valuable equipments, complicated operation, long time detection, and can’t test in time, thus they suit to use as identified method. The rapid test paper produced by DaLian Puruikang Company is sensitive and accuracy, it can test in time, don’t restrict by site, people, so it can use as a screening method in pig farm, market, slaughterhouse, etc.
(3) It is necessary to built up a detection system, which combines the screen method and the determination method. The rapid test paper detection could as a screening method, and the HPLC, GC-MS and ELISA detection could as determination method. This system will be the effective measure to control Clenbuterol-HCl from origin in animal production.