URINARY ORGAN-SPECIFIC NEOANTIGEN - A POTENTIALLY DIAGNOSTIC-TEST FOR COLORECTAL

2020-04-05 18:15:56

patients cancer specific effluent BAC

责任者: TOBI, M;DARMON, E;ROZEN, P;HARPAZ, N;FINK, A;MALIAKKAL, B;HALLINE, A;MOBARHAN, S;BENTWICH, Z 单位: TEL AVIV MED CTR & SCH MED,DEPT GASTROENTEROL,TEL AVIV,ISRAEL.;KAPLAN HOSP,BEN ARI IMMUNOL INST,IL-76100 REHOVOT,ISRAEL.;UNIV ILLINOIS,DIV GASTROENTEROL,CHICAGO,IL.;LOYOLA UNIV,MED CTR,MAYWOOD,IL 6015 来源出处: DIGESTIVE DISEASES AND SCIENCES, v 40, JUL 1995, p 1531- 1537 摘要: Urinary organ-specific neoantigen from colorectal cancer patients has been used to make a monoclonal antibody, BAC 18.1. In this study we assessed the potential of this antibody for the diagnosis of colorectal cancer. We evaluated binding in both urine and effluent samples and compared it with effluent carcinoembryonic antigen standardized for both volume (nanograms per milliliter) and protein. Urinary organ-specific antigen as detected by BAC 18.1 was significantly greater in 29 cancer patients (A(405): 0.717 +/- 0.500) vs 27 controls [0.121 +/- 0.273 (P < 0.05)]. Considerable overlap of binding of BAC 18.1 was observed in the colonic effluent of patients with CRC (N = 13), adenomas (N = 26), inflammatory bowel disease (N = 8), or having a normal colonoscopic examination (N = 24). CEA levels (nanograms per milliliter) were significantly elevated in the effluent samples of patients with a past history of colorectal cancer, as compared to that of normal individuals (P < 0.05). The presence of the Mr 30,000 organ-specific neoantigen in colonic effluent was also demonstrated by western blot. Organ-specific neoantigen originates in the colon and is excreted into the urine, so the BAC 18.1 binding levels in the urine may be a diagnostic aid for CRC. 关键词: MONOCLONAL ANTIBODY; COLORECTAL CANCER; LEUKOCYTE ADHERENCE INHIBITION; CARCINOEMBRYONIC ANTIGEN CARCINOEMBRYONIC ANTIGEN; LEUKOCYTE ADHERENCE INHIBITION; CARCINOEMBRYONIC ANTIGEN CEA; MONOCLONAL-ANTIBODIES; COLONIC EFFLUENT; HIGH-RISK; PROTEINS; ADNAB-9; MONOCLONAL-ANTIBODIES; COLONIC EFFLUENT; HIGH-RISK; PROTEINS; ADNAB-9; TUMORS; PLASMA; FECES TUMORS; PLASMA; FECES