KINETICS OF INHIBITION OF METHANE OXIDATION BY NITRATE, NITRITE, AND AMMONIUM IN

2020-04-05 17:52:08

soil concentrations NH4 CH4 inhibitory

责任者: DUNFIELD, P;KNOWLES, R 单位: MCGILL UNIV,DEPT NAT RESOURCE SCI,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA. 来源出处: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v 61, AUG 1995, p 3129- 3135 摘要: The kinetics of inhibition of CH4 oxidation by NH4+, NO2-, and NO3- in a humisol was investigated. Soil slurries exhibited nearly standard Michaelis-Menten kinetics, with half-saturation constant [K-m(app)] values for CH4 of 50 to 200 parts per million of volume (ppmv) and V-max values of 1.1 to 2.5 nmol of CH4 g of dry soil(-1) h(-1). With one soil sample, NH4+ acted as a simple competitive inhibitor, with an estimated K-i of 8 mu M NH4+ (18 nM NH3). With another soil sample, the response to NH4+ addition was more complex and the inhibitory effect of NH4+ was greater than predicted by a simple competitive model at low CH4 concentrations (10 mM concentrations, which also altered soil pHs. Similar concentrations of NaCl were also inhibitory of CH4 oxidation, so there may be no special inhibitory mechanism of nitrate per se. 关键词: ENDOTHELIUM; BLOOD-BRAIN BARRIER; DRUG DELIVERY; ANTISENSE OLIGODEOXYNUCLEOTIDE; RNASE H; PHARMACOKINETICS; PHOSPHOROTHIOATE; OLIGODEOXYNUCLEOTIDE; RNASE H; PHARMACOKINETICS; PHOSPHOROTHIOATE; PEPTIDE NUCLEIC ACID PEPTIDE NUCLEIC ACID; SOILS; CH4; FERTILIZATION; TEMPERATE; NITROGEN; FOREST; FLUXES; WATER; BATH BATH