IMPROVED VIRUS SAFETY AND PURITY OF A CHROMATOGRAPHICALLY PRODUCED FACTOR-IX CON

2020-04-05 16:59:04

factor effect Of purification IX

责任者: HOFFER, L;SCHWINN, H;BIESERT, L;JOSIC, D 单位: OCTAPHARMA PHARMAZEUT PROD MBH,A-1100 VIENNA,AUSTRIA.;GEORG SPEYER HAUS,D-60596 FRANKFURT,GERMANY. 来源出处: JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, v 669, JUL 21 1995, p 187- 196 摘要: A virus removal system based on tangential flow filtration was introduced into a Factor IX production process. Beside the intended virus reduction potency of filter membranes, an additional purification effect could be achieved. This purification effect was evaluated in detail by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis and size-exclusion HPLC. High-molecular-mass impurities were retained by the membrane, thus increasing the specific activity of the product. 关键词: ASSAY METHODS; CHITIN; CORNEA; FUNGAL KERATITIS (KERATOMYCOSIS); HUMAN-PLASMA; FACTOR-X