EFFECTS OF THE PASTEURELLA-MULTOCIDA TOXIN ON OSTEOBLASTIC CELLS IN-VITRO

2020-03-26 16:52:18

cell toxin ROS osteoblastic

责任者: STERNERKOCK, A;LANSKE, B;UBERSCHAR, S;ATKINSON, MJ 单位: HANNOVER SCH VET MED,INST PATHOL,W-3000 HANNOVER,GERMANY.;GSF MUNICH,INST PATHOL,NEUHERBERG,GERMANY. 来源出处: VETERINARY PATHOLOGY, v 32, MAY 1995, p 274- 279 摘要: Pasteurella multocida toxin induces localized osteolysis in the turbinate bones of swine. Osteolysis appears to be due to an increased level of osteoclastic bone resorption, although osteoblast activity may also be impaired. We studied the effects of purified toxin on the osteoblastic phenotype of the ROS 17/2.8 rat osteoblastic osteosarcoma cell line. Treatment of both embryonic bovine lung cells and a nonosteoblastic rat osteosarcoma cell Line (ROS 25/1) with nanomolar doses of toxin produced marked cytotoxic actions. In the osteoblastic ROS 17/2.8 cells, this level of toxin reduced expression of an osteoblastic marker (alkaline phosphatase), was associated with matrix mineralization, but had no cytopathologic action. The osteoblastic cell population may be resistant to a direct cytotoxic effect but is nevertheless a target for toxin action. 关键词: ALKALINE PHOSPHATASE; OSTEOBLAST; PASTEURELLA MULTOCIDA TOXIN; RHINITIS ATROPHICANS ATROPHICANS; DERMONECROTIC TOXIN; PARATHYROID-HORMONE; BONE-RESORPTION; POTENT MITOGEN; ATROPHIC RHINITIS; SEROTYPE-D; PIGS; PURIFICATION; CULTURE; MITOGEN; ATROPHIC RHINITIS; SEROTYPE-D; PIGS; PURIFICATION; CULTURE; STIMULATION STIMULATION