IN-VITRO STUDY OF THE ANTILEISHMANIAL ACTIVITY OF BIODEGRADABLE NANOPARTICLES

2020-03-26 16:28:47

The cells drug nanoparticles carriers

责任者: VENIERJULIENNE, MC;VOULDOUKIS, I;MONJOUR, L;BENOIT, JP 单位: UNIV PARIS 06,INSERM,U313,PARASITOL EXPTL LAB,F-75013 PARIS,FRANCE.;FAC MED IRAKLION,DEPT GEOG MED & MICROBIOL,GR-71110 IRAKLION,GREECE. 来源出处: JOURNAL OF DRUG TARGETING, v 3 1995, p 23- 29 摘要: Leishmania are obligate intracellular parasites, responsible for leishmaniasis. Leishmaniasis are transmitted via insect vector to vertebrate hosts including humans. The infection was reproduced in vitro with promastigotes which can infect murine resident peritoneal cells. Amphotericin B was incorporated into poly(D, L-lactide-co-glycolide) nanoparticles, biodegradable drug carriers, to allow specific targeting inside the cell. The interaction of the drug with infected cells was determined by exposing macrophage cultures to drug carriers. The toxic effects of polymeric drug carriers were defined prior to exposing cells to drug-loaded nanoparticles. For contact times up to 4h, cells tolerated polymer concentrations of 0.01%. The viability of parasites after treatment was determined. Infected macrophages were incubated at 26 degrees C (which allows the transformation of amastigote to promastigote) along with loaded and unloaded nanoparticles, as well as the free drug alone, and a count of the parasites in the medium was recorded. Anti-leishmanial activity was observed with drug-free nanoparticles. This activity may arise through the release of hydrogen peroxide following the activation of macrophages. The incorporation of amphotericin B did not enhance this effect. Interestingly, trehalose, a cryoprotector of the freeze-dried nanoparticles, altered parasite growth and activated macrophages. 关键词: AMPHOTERICIN B; LEISHMANIA; MACROPHAGES; NANOPARTICLES; PLAGA; TREHALOSE; VISCERAL LEISHMANIASIS; MACROPHAGES; PHAGOCYTOSIS; LIPOSOMES; AMPHOTERICIN; NEUTROPHILS; FORMS; ASSAY; ACID AMPHOTERICIN; NEUTROPHILS; FORMS; ASSAY; ACID