Photochemical ODN manipulation based on reversible DNA photoligation mediated by

2020-03-11 21:11:28

SCI ODN alpha Japan photoligation

责任者: Ogino, M.;Ogasawara, S.;Ami, T.;Yoshimura, Y.;Fujimoto, K. 单位: Sch. of Mater. Sci., Japan Adv. Inst. of Sci. & Technol., Ishikawa, Japan 来源出处: Journal of Photopolymer Science and Technology(J. Photopolym. Sci. Technol. (Japan)),2005//,18(4):507-12 摘要: We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using modified photoresponsive base. An efficient photoligation was produced by photoirradiation of an ODN containing α-5-cyanovinyldeoxyuridine (αCU) at the 3 end with an ODN containing thymine at the 5 end in the presence of a template ODN. This photoligation method is a new, efficient way to synthesize branched ODNs 关键词: DNA;molecular biophysics;nanotechnology;photochemistry;proteins;photochemical ODN manipulation;reversible DNA photoligation;template-directed photoligation;alpha-5-cyanovinyldeoxyuridine;thymine;photoresponsive base;oligodeoxynucleotides;photoirradiation