Conformational change of adsorbed and desorbed bovine serum albumin on nano-size

2020-02-14 23:54:57

magnetic particles change BSA desorbed

责任者: Peng, Z.G.;Hidajat, K.;Uddin, M.S. 单位: Dept. of Chem. & Environ. Eng., Nat. Univ. of Singapore, Singapore 来源出处: Colloids and Surfaces B (Biointerfaces)(Colloids Surf. B, Biointerfaces (Netherlands)),2004/01/01,33(1):15-21 摘要: Adsorptions of bovine serum albumin (BSA) on nano-sized magnetic particles with and without the presence of carbodiimide were studied. Desorption of BSA from magnetic particles were carried out in either NaOH or Na2HPO44 solutions. The structures of native BSA, adsorbed BSA on magnetic particles, and desorbed BSA were studied by several methods, circular dichroism (CD), fluorescence spectroscopy and differential scanning calorimetry (DSC). The magnitude of conformational changes of protein was determined by calculating the α-helix content from the circular dichroism (CD) spectra and by evaluating fluorescence spectrum and DSC thermograms. Adsorbed BSA on magnetic particles shows no thermal transition with respect to the native BSA. The structural change of BSA when desorbed by Na2HPO4 solution is much smaller in comparison to that when desorbed by NaOH solution. Hence, this indicates that BSA could be desorbed from nano-sized magnetic particles using Na2HPO4 without much conformational change 关键词: adsorption;bioluminescence;biothermics;circular dichroism;desorption;differential scanning calorimetry;fluorescence spectroscopy;magnetic materials;molecular biophysics;molecular configurations;nanostructured materials;proteins;conformational change;adsorbed bovine serum albumin;desorbed bovine serum albumin;nano-sized magnetic particles;adsorptions;carbodiimide;desorption;circular dichroism;fluorescence spectroscopy;differential scanning calorimetry thermograms;protein;α-helix content;structural change