Functionalized semiconductor nanocrystals for ultrasensitive detection of peptid

2019-08-29 17:51:14

mL GA detection mu CdS

责任者: Chen, Xudong;Wang, Xinbo;Liu, Lin;Yang, Dacheng;Fan, Li 单位: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China 来源出处: Analytica Chimica Acta,2005,542(2):144-150 摘要: Semiconductor CdS nanoparticle have been prepared and modified with thiovanic acid. The functionalized nanoparticles are water-soluble. They were used as the fluorescence probes in the ultrasensitive detection of peptides. This method is based on the fluorescence enhancement of functionalized nano-CdS in the presence of peptide with mercapto groups (GN-9) and the fluorescence quenching of functionalized nano-CdS in the presence of peptide (GA-8 and MT-25). Excitation and emission wavelengths were 360 and 530 nm, respectively. Under optimum conditions, the calibration graphs are linear over the range of 0.15-3.5, 0.2-4.0, and 0.2-3.8 μg ml-1 for GN-9, GA-8 and MT-25, respectively. The corresponding detection limits were 0.010 μg ml -1 for GN-9, 0.018 μg ml-1 for GA-8 and 0.022 μg ml-1 for MT-25, respectively. This method has been proved to be a simple, rapid and sensitive method. © 2005 Elsevier B.V. All rights reserved. 关键词: Nanostructured materials;Semiconductor materials;Amino acids;Cadmium sulfide;Probes;Fluorescence;Quenching;Peptides;Nanoparticles;Ultrasensitive detection;Mercapto groups